FastQCFastQC Report
Sun 14 Apr 2019
SRR6576323_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576323_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364909
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7010.19210268861551785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.14387148576768455No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4760.13044348042936732No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4620.1266069074755624No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4180.11454910676360408No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3860.10577979715490711No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3740.10249130605164575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.047.1011
AACGCAG14600.040.237796
ATCAACG14700.039.6497843
TCAACGC14800.039.381884
CAACGCA15150.038.7770165
TACATGG22550.035.4355242
GTACATG23350.034.6953851
ACATGGG22950.033.9986653
ACGCAGA17350.033.8600437
TATCAAC17550.032.94312
CGCAGAG18000.032.6373188
CATGGGA14000.031.559954
ATGGGAG5600.030.2128315
GCAGAGT20000.029.6085789
ATAGCAC1005.310499E-528.2025073
GTATTAG2101.3878889E-924.6719551
CATGGGG6950.024.3474884
AGAGTAC18450.023.68991710-11
ATGGGAT3800.023.4988695
TAGCGCG2453.3651304E-1023.0224534
Produced by FastQC (version 0.11.2)