FastQCFastQC Report
Sun 14 Apr 2019
SRR6576324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335106
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8240.2458923445118858No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5370.1602478021879644No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.14383508501787493No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4240.12652712872941696No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3810.11369536803280157No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3750.11190488979606454No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3720.11100965067769601No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3600.10742869420422195No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3520.10504138988857256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.041.3650321
TACATGG23450.038.271242
ACTATCC500.001702536537.589978
GTACATG24050.037.5118261
ACATGGG24250.036.621163
ATGGGAT3350.035.0652735
GTCCTAA550.00271782634.1727031
CATGGGT5600.033.5624774
ATCAACG14500.032.72923
GGTACCT3450.032.686938
AACGCAG14550.032.616736
TCAACGC14650.032.394094
ATGGGTA3650.032.1831975
CAACGCA14950.031.7440415
GGGTACC3650.030.8958687
CATGGGA14500.029.4886864
GACTAAT804.7812014E-429.3671651
ACGCAGA16450.028.8494457
ATGGGAG4950.028.4772535
CGCAGAG16700.028.4175688