FastQCFastQC Report
Sun 14 Apr 2019
SRR6576324_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576324_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335106
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7330.21873675792137412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.15726367179340267No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4720.1408509546233132No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4070.12145410705866203No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4040.12055886794029352No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4000.11936521578246884No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3740.11160647675660836No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3740.11160647675660836No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3580.10683186812530959No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3500.10444456380966022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12100.041.987411
TAATACC704.630605E-640.279134
GTACATG22750.037.219611
TACATGG22600.036.8037152
ACATGGG22550.035.0097963
AACGCAG14350.034.7120936
CATGGGG7100.034.416914
ATCAACG15250.032.663513
TCAACGC15700.031.7272974
ACGCAGA15800.031.5311997
CAACGCA15850.031.4270385
GTCTTAG1201.633689E-731.3609681
CGCAGAG16300.030.5822338
GCAGAGT17900.027.8486299
GTCCATA856.770948E-427.671441
CATGGGA14050.027.426124
TACCGTC1205.8276473E-627.4162777
ATAATAC1205.834414E-627.4121843
TATCAAC19250.027.0968672
ACCGTCG1406.313476E-726.8728038