FastQCFastQC Report
Sun 14 Apr 2019
SRR6576325_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576325_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413754
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11400.2755260372105164No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7060.17063279146546015No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6520.15758155812390937No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6440.15564804207330926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.15226438898475905No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6180.1493641149088589No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG6180.1493641149088589No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5980.1445303247823586No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5920.1430801877444085No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5750.13897146613688327No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5650.1365545710736331No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5620.13582950255465806No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT5300.12809543835225762No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5190.12543685378268246No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC5120.12374502723840736No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4740.11456082599805681No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT4500.10876027784625646No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG4320.10440986673240621No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4230.10223466117548108No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC4190.10126790315018103No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4150.10030114512488096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGG250.001700827537.60415660-61
GTATCAA15000.036.491841
TCTACAC1256.2555046E-933.835563
ATGGGTA5200.033.4419635
GTACATG24450.033.0024571
TCAACGC16150.032.3030744
ATCAACG16100.031.8157043
CAACGCA16250.031.8150585
ACATGGG25000.031.7678363
GGGTACC3850.031.7399337
CTCGATA300.00415806431.33679864-65
CGCGTCC300.004162986331.32922416-17
AACGCAG16750.031.1459486
TACATGG26100.030.9766042
CATGGGT7450.030.281574
GGGCCTA953.746798E-529.6839057
GACATAT804.6654572E-429.4923271
GTACTGT804.775942E-429.37476
CATGGGG7200.029.3746994
CTAAGAC1452.654815E-829.1685893
Produced by FastQC (version 0.11.2)