FastQCFastQC Report
Sun 14 Apr 2019
SRR6576328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393301
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7700.19577880554587962No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4620.11746728332752776No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4530.11517895962634217No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4010.10195753379726978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCC405.672669E-446.9991074
GTATCAA12300.036.691561
GTACATG26000.035.800731
ACATGGG25350.035.782363
TACATGG26000.035.0774882
AACGCAG14650.030.477246
ATCAACG14700.030.0538543
TCAACGC14700.030.0538544
CATGGGA13800.029.970454
CAACGCA15000.029.7661045
CATGGGT5550.028.7922464
CATGGGG8100.028.4315594
TAATACT1904.456524E-1027.2100124
ATGGGAT4200.026.8566345
CATGGGC4650.026.2790724
ATGGGTA3400.026.264215
CGCAGAG17250.025.8835688
TATCAAC17200.025.6920932
GTATAGA1101.0150432E-425.6423971
CTAGTAC1101.01679965E-425.6358783
Produced by FastQC (version 0.11.2)