FastQCFastQC Report
Sun 14 Apr 2019
SRR6576330_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576330_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265942
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5020.18876296335291154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.11995096675214896No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2890.10867031157169608No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2820.10603815869625707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAT200.002161763870.494938
TACATGG14550.038.135612
GTATCAA8050.037.4765241
GTACATG15100.037.1488341
ACATGGG14950.036.4861833
ATGGGTA2150.034.9873855
CATGGGT3800.033.411184
GGGTACC2450.032.6098987
TGGGTAC2200.032.0552026
AACGCAG10050.030.8750616
CAACGCA9950.030.712865
ATCAACG9650.030.6990473
TCAACGC9650.030.6990474
CTGGCGT804.757381E-429.3894634
CATGGGA8200.027.5257424
TACGCTA2053.6379788E-1127.5102149
ACGCAGA11300.027.449357
GGTACCT2750.027.3434858
CGCAGAG11600.027.1445988
GTAGTAA700.00869832726.9362531
Produced by FastQC (version 0.11.2)