FastQCFastQC Report
Sun 14 Apr 2019
SRR6576332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281357
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4990.177354748593424No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3150.11195740642671054No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.10627068102090936No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2820.10022853527724564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG250.005231943456.3999985
GTATTAC654.786125E-850.6153871
GTATCAA8400.047.5595251
TACATGG17650.040.742212
GTACATG17800.040.662921
TAGATGG853.9414772E-738.705885
CAACGCA10350.038.5990335
AACGCAG10500.038.4952356
ACATGGG18150.038.0661163
ATCAACG10600.037.688683
TCAACGC10750.037.64
CATGGGA10850.033.7880174
TATCAAC12200.032.7459032
CTGGTCG1052.0580446E-631.3333369
ATAGGTG600.004155691231.3333345
GTAATAG600.004155691231.3333341
ACGCAGA12900.031.3333327
CGCAGAG13200.030.621218
GCAGAGT13900.029.4172639
TGTAGCG804.771816E-429.37594
Produced by FastQC (version 0.11.2)