FastQCFastQC Report
Sun 14 Apr 2019
SRR6576332_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576332_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281357
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5830.20721005697388017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.1496319622401433No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3790.13470430804991523No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3790.13470430804991523No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3220.11444534879174856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG17400.039.290431
GTATAAG1101.7316779E-938.5760541
ACATGGG17800.038.0206033
TACATGG18150.037.8053052
GTATCAA7850.035.4364621
ATGGGAG3800.032.1563155
CATGGGG7050.031.9984284
GTATTGG753.2093548E-431.432341
CATGGGA9200.030.1398244
ATGGGTA1951.8189894E-1128.9216565
CATGGGT3750.028.8252494
TGGGAGA2700.027.8504836
AACGCAG10200.027.6456996
ATCAACG10050.027.1230453
CAACGCA10250.026.5938155
CATGGGC3550.026.477574
TCAACGC10500.025.9606294
GGTACCT2008.058123E-1025.8487288
ATGGGGG3350.025.2524915
GGGAGAT1901.3244062E-824.7356267
Produced by FastQC (version 0.11.2)