FastQCFastQC Report
Sun 14 Apr 2019
SRR6576333_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576333_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258179
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6500.2517633115009354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.16112851936059866No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3570.1382761572397445No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3130.12123371769198889No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2760.10690257534501257No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2760.10690257534501257No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2640.10225463728653376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8850.044.696761
TACATGG17600.039.515072
GTACATG17600.039.331881
GGGTACC2850.037.9224787
ACATGGG18150.037.540943
ATGGGTA3300.037.0231325
CATGGGT5000.036.65294
TGGGTAC2950.036.636976
AACGCAG10750.035.8442156
GGTACCT3050.035.4632348
CTATATA959.427258E-734.6248744
TCAACGC11200.034.4040534
GTATAAC550.002687442834.2481651
ATCAACG11300.034.099593
CAACGCA11400.033.800475
CGCAGAG11950.032.2698028
CATGGGA9050.032.192664
ACGCAGA12100.031.8450727
GTATTAC600.004115799431.3941541
TATATAG1401.8824721E-830.2084355
Produced by FastQC (version 0.11.2)