FastQCFastQC Report
Sun 14 Apr 2019
SRR6576334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449713
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12600.28017869174340077No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6640.1476497232679509No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6600.14676026710368612No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5780.12852641573625845No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5700.12674750340772892No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5400.1200765821757432No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4900.10895838012243363No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4790.10651237567070554No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4590.10206509484938173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10250.044.491681
GCGTAAG250.001702057837.59897664-65
ACATGGG24200.035.3461463
CATGGGT7000.034.9133384
GTACATG25000.034.60261
ATCAACG12700.034.4163863
TACATGG26150.032.9010932
CAACGCA13350.032.7406855
TCAACGC13500.032.3768964
ATGGGTA5100.032.2540255
AACGCAG14050.031.7784946
GGTACCT5200.028.9222898
CATGGGG10950.028.3279954
CTGTATA856.8106747E-427.6463054
ACGCAGA16350.027.5955817
CGCAGAG16450.027.4278268
ATACCTT1205.834052E-627.415926
GGTATCA5050.026.9983711
GTAGCAC909.51015E-426.1104013
TATCAAC17450.025.8646582