FastQCFastQC Report
Sun 14 Apr 2019
SRR6576335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406302
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12540.30863741748748463No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6830.16810155992340672No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6330.15579544279870639No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5530.13610565539918582No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5530.13610565539918582No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5410.13315218728925773No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4850.11936933610959335No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4610.11346239988973719No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4520.11124729880729112No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4370.10755546366988103No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4250.10460199555995295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10350.041.7810061
ACATGGG23550.039.9083333
GTACATG24950.038.620221
TACATGG25750.037.2424132
CATGGGG11050.036.9982764
AACGCAG11600.034.4338386
CATGGGT6450.034.2422754
CAACGCA12050.033.927885
TCAACGC11950.033.8185584
ATCAACG12050.033.5379033
ATGGGTA4700.030.994765
ACGCAGA13450.029.6975867
CGCAGAG13700.029.4986678
GGTACCT4950.029.4293738
GGGTACC5050.028.8466137
TGCCCGC1154.185098E-628.6109051
ATGGGGA7100.028.4599825
GCATTAA1057.40607E-526.8592191
GTATGAT1057.40607E-526.8592191
ATAGTAC700.00883669526.8526088