FastQCFastQC Report
Sun 14 Apr 2019
SRR6576335_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576335_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406302
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11010.2709806990859016No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6220.1530880970312723No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5960.14668891612642812No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5370.13216769791928173No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4940.12158443719203942No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4760.1171542350271473No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4720.11616974565717127No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4720.11616974565717127No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4560.11223178817726717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4330.106570974299905No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4140.10189464979251886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10850.041.6541981
GTACATG22450.036.4880071
ACATGGG22700.036.023983
TACATGG23300.035.5040972
CATGGGG10150.034.2636764
TCAACGC13350.033.7954524
AACGCAG13750.033.1500246
CAACGCA13700.032.9320645
ATCAACG13850.032.57543
GTGTAAT1605.820766E-1132.3660661
GTACTAT600.004124265631.3852791
ACGCAGA14900.030.276097
GTACTTT11200.029.844041
ACCTAAG804.729402E-429.4236981
CGCAGAG15200.029.3874668
CATGGGT6400.029.3729974
TATAGAG1005.317648E-528.1980825
GGTACCT4350.028.1038788
ATAGACG856.791578E-427.6587928
TAATCGT700.00880587226.87185394