FastQCFastQC Report
Sun 14 Apr 2019
SRR6576336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676244
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17960.265584611471599No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11480.16976121045066572No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10790.15955779274936266No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10070.14891074819148117No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9600.14196059410508632No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9400.13900308172789705No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9000.13308805697351844No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8470.12525064917396678No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA8450.12495489793624785No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC8360.12362401736651267No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT7980.118004743849853No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC7840.11593448518582049No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7780.11504723147266371No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7400.10942795795600405No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7010.10366080882048491No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT6980.1032171819639065No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6910.10218205263189026No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG6820.10085117206215509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.053.856281
TCAACGC18850.038.39674
CAACGCA19000.038.093575
TTAGGTA2500.037.598854
ATCAACG19250.037.598853
CTTAGGT2500.037.598853
AACGCAG19700.037.2171336
AGGTATA2900.035.654086
TAGGTAT2700.034.8137475
TATAACG550.00271564534.1832962
GTCTTAG3250.033.2875941
TATCAAC24150.030.7508142
TACATGG31650.030.2951492
GTACATG32150.029.992371
GTATAGT3300.029.9081738
ACATGGG31800.029.854433
GTGTAGA1751.6916601E-1029.5661
TAGTTAA804.7796848E-429.37409894
TATAGTA3200.029.3740989
ACCTAAG3850.029.3216511
Produced by FastQC (version 0.11.2)