FastQCFastQC Report
Sun 14 Apr 2019
SRR6576337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374669
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13080.34910814612364516No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6930.1849632609049588No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6510.17375336630465824No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6340.1692160280140604No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6260.16708080999495556No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5590.14919835908495233No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5080.13558634421315882No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4910.13104900592256097No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4840.1291806901558442No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4540.121173622584201No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4500.12010601357464856No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4420.1179707955555437No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4360.11636938204121505No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4350.11610247978882694No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4330.11556867528405071No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4280.11423416402211019No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4170.111298239245841No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4140.11049753248867668No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG4040.1078285099647956No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG3910.1043587806837502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT651.4118133E-436.1532336
GTATCAA11150.035.4121971
ACATGGG24200.034.3754463
CCGTATG550.002714909134.1812369
TACATGG24650.033.94312
CATGGGT7150.033.5239034
GTACATG25150.033.4551851
ATGGGTA5200.030.7302465
GGTACCT5100.029.4896958
ATCAACG13550.028.0954633
TCAACGC13750.028.0286144
AACGCAG14050.027.764656
TAATAGA856.8081345E-427.6465856
CTATACT856.8081345E-427.6465854
CAACGCA14050.027.0956235
TGGGTAC5950.026.8566846
GGGTACC5800.025.9305937
CATGGGC4650.025.2683874
GTACCTG6150.025.2190849
GTAATAC950.001303074624.736423
Produced by FastQC (version 0.11.2)