FastQCFastQC Report
Sun 14 Apr 2019
SRR6576339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390213
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12300.3152124608867465No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7160.18348953007716298No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7110.18220817861014368No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6580.16862585305973918No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6130.15709368985656552No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5480.14043612078531467No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5150.13197920110298733No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5070.12992903875575645No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5020.12864768728873716No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4750.12172838936683299No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4620.11839687555258283No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4620.11839687555258283No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4310.11045249645706319No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4260.10917114499004389No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4190.10737725293621689No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4110.10532709058898601No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC3940.10097049560112041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8250.045.0338671
GTTATAC651.4119719E-436.1530083
ACATGGG21350.033.4605833
GTACATG22350.033.036061
TACATGG22500.032.3977852
TATATAC902.5899793E-531.3326073
CATGGGT5350.030.746954
ATCAACG11850.030.5393753
TCAACGC12150.029.7853184
CATGGGG10650.029.5673894
CAACGCA12150.029.3984975
ACTGCGT804.775811E-429.374328
AACGCAG12550.028.8359876
ATAGCAC1503.6854544E-828.1993483
ATAACGC1354.622416E-727.8512083
ATGGGTA4250.027.646425
GGTATCA4400.026.7210461
GTATAAC909.472152E-426.1272451
ACGCAGA14750.024.5350237
CGCAGAG15100.024.2775848
Produced by FastQC (version 0.11.2)