FastQCFastQC Report
Sun 14 Apr 2019
SRR6576341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453919
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8630.19012202617647642No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5700.12557306479790448No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.12182790321621259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12350.044.9295231
AACGCAG14450.038.3788226
CAACGCA14750.037.598245
TACATGG28200.037.5189062
GTACATG28350.037.4862631
ATCAACG14850.037.345053
TCAACGC15000.036.97164
ACATGGG28400.036.7377173
CATGGGT6650.032.5097544
TATCAAC17750.031.5257472
CATGGGA15850.030.8376714
ACGCAGA18350.030.2220157
CGCAGAG18700.029.6563638
ATGGGTG2800.028.5343785
CCTAATA856.789649E-427.6612
ATGGGTA4350.027.0102275
ATGGGAT5100.026.7242375
ATGGGAG8100.026.6901075
GCAGAGT21600.025.8923059
TAATACC1301.0863332E-525.3065074
Produced by FastQC (version 0.11.2)