FastQCFastQC Report
Sun 14 Apr 2019
SRR6576341_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576341_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453919
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8070.17778502331913842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.13592733505317028No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5440.11984517061414042No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4900.10794877500170735No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4600.10133966632813343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12600.051.6729971
GTACCGT450.00101299141.7804226
ATCAACG15650.041.747053
AACGCAG15800.040.4582566
TCAACGC16350.039.9597174
CAACGCA16450.039.71685
TATCAAC18650.035.535762
GTACATG26550.034.118651
CTAATCC702.182116E-433.5735553
ACGCAGA19050.033.3055277
TACATGG27350.033.168462
ACATGGG27050.032.6675033
CGCAGAG19500.032.295928
CATGGGT6550.030.1393134
GCAGAGT21700.029.2382629
GGGTACC3750.028.8253157
CATGGGA14500.026.9051514
ATGGGAG7550.026.147355
ATGGGTA4500.025.0682535
CATGGGG9900.024.6884334
Produced by FastQC (version 0.11.2)