FastQCFastQC Report
Sun 14 Apr 2019
SRR6576342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416462
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8020.19257459263990473No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5920.14214982399354562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.12630203956183278No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4770.11453626021101565No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4680.11237519869760025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13100.046.296641
TACATGG25850.038.9210242
GTACATG26150.038.8340871
ACATGGG26450.037.660013
ATCAACG16400.036.672153
AACGCAG16550.036.3397756
CAACGCA16800.035.519325
TCAACGC17150.034.794444
CATGGGT5750.033.50324
TATCAAC18850.032.4237372
ATGGGAG6900.031.3241275
AGTGCGT600.00416394731.3241278
CATGGGA14900.031.2190154
ACGCAGA19700.030.767617
CGCAGAG20000.030.0711618
CATGGGG9350.027.6389354
GCAGAGT22050.027.4885229
TATAAGC1205.8175647E-627.4250762
CTTATCC950.001305422924.7295743
TTATTAC950.001305422924.7295743
Produced by FastQC (version 0.11.2)