FastQCFastQC Report
Sun 14 Apr 2019
SRR6576342_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576342_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416462
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7680.18441058247811323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.14551147523663624No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5200.12486133188622252No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4450.10685248594109427No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4250.1020501270223934No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4240.10181000907645836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11500.040.1412471
GTACATG28500.037.683621
TACATGG29150.037.0772742
ACATGGG29250.036.468553
CATGGGT7000.032.2226874
CATGGGA15200.032.152024
AACGCAG15450.030.4151576
ATGGGTG2700.029.5871875
ATCAACG15700.029.0329113
TCAACGC16150.028.5149154
CAACGCA16300.027.9642165
CATGGGG9200.027.071144
GTCTATA909.3849364E-426.169181
ACTATAG909.517595E-426.1063423
TATCAAC18450.025.2149072
ACGCAGA18950.024.5525517
CGCAGAG19200.024.2474178
ATGGGAG7200.024.1483675
GTATAAT2002.2359018E-823.5522611
TATACCC1000.001758672923.4957085
Produced by FastQC (version 0.11.2)