FastQCFastQC Report
Sun 14 Apr 2019
SRR6576343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198494
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6490.3269620240410289No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3550.17884671576974617No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3550.17884671576974617No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3300.16625187663103166No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3030.15264945036122No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2730.13753564339476254No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2400.1209104557316594No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2360.11889528146946508No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2300.11587252007617357No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2260.11385734581397927No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2190.1103307908551392No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2180.10982699728959062No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2180.10982699728959062No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2140.1078118230273963No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2060.10378147450300765No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2050.10327768093745905No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2050.10327768093745905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGG250.00522457356.4139142
CATATAG250.00522457356.4139141
GTATCAA5350.038.663741
TTATTGG500.001698681337.59984
TATAGGC651.409325E-436.1536523
GTACATG10350.034.9748081
GTATAGA550.00270834234.190251
ATATAGG702.1753648E-433.5797122
CACTCTA1554.1836756E-1133.354669
ACATGGG10750.032.7905243
TACATGG10950.032.629052
TATTATA902.5788562E-531.341062
AACGCAG6700.030.1640176
ATCAACG7050.029.3331783
TCAACGC6950.029.0789814
CCTTAAG650.00613467428.9302121
CAACGCA7000.028.8712735
CCTATAA856.786298E-427.6538771
TACTCCC856.796308E-427.646915
ATATTAT1057.378802E-526.863771
Produced by FastQC (version 0.11.2)