FastQCFastQC Report
Sun 14 Apr 2019
SRR6576343_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576343_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198494
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6100.30731407498463426No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4300.21663123318588975No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3510.17683154150755187No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3070.15466462462341432No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2960.1491228954023799No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2520.12695597851824236No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2400.1209104557316594No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2350.11839148790391649No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2240.1128497586828821No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2230.11234596511733351No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2230.11234596511733351No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2180.10982699728959062No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2090.10529285519965338No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2070.10428526806855623No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2030.1022700938063619No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2000.10075871310971617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAATA250.005230842656.3968242
TACTCCG450.001012206941.775435
GTATCAA5300.039.9537471
GTCCTAC757.351513E-637.645311
GCGTTCC250.001701155737.5978864-65
GTATACA500.001699106537.597884
TACTAAA651.4097475E-436.151812
GTATAAG550.002695648534.2230071
CTATACC550.002712368534.1798934
TAGTTCC702.1760163E-433.57799594
GTACATG11000.032.511861
TACATGG11450.032.426122
AACGCAG6900.032.012696
ACATGGG11600.032.006823
CATGGGT3250.031.8135954
GGTACCT1950.031.331578
CAACGCA7050.030.6649385
ATCAACG7100.030.448993
ATGGGTA2200.029.9074085
TCAACGC7350.029.4133114
Produced by FastQC (version 0.11.2)