FastQCFastQC Report
Sun 14 Apr 2019
SRR6576344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179119
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6490.3623289544939398No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3950.2205237858630296No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3900.2177323455356495No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3110.17362758836304357No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2850.15911209866066695No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2680.14962120154757452No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2440.13622228797614994No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2310.1289645431249616No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2260.12617310279758148No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2230.12449823860115343No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2220.1239399505356774No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2190.12226508633924932No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2050.11444905342258499No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2020.11277418922615691No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2020.11277418922615691No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG2010.11221590116068088No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2000.11165761309520486No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1980.11054103696425283No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1940.10830788470234873No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG1920.10719130857139667No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1890.1055164443749686No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1870.10439986824401655No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1800.10049185178568439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA301.3697469E-462.6506653
CTACACT250.00523413556.3855974
ACACTAA250.00523413556.3855976
CGCTAAA352.9345052E-453.7005655
ATGGGAT1051.1314114E-940.275435
ATATAGG608.7930275E-539.16762
ACAATGC1055.0824383E-835.800388
TGCCCGC801.153015E-535.250841
GTACATG12500.034.9688341
TAATACC550.002714314534.1730884
ATAATAC550.002714314534.1730883
CATGGGG5400.033.9357764
ACATGGG12950.033.3814353
TACATGG13000.033.262332
GTATCAA6250.033.0887871
CATTAGG300.004162393531.32533310-11
ATGCACC600.00415688831.3253334
CCGCTAA650.006148163728.915694
GTATATA650.006148163728.915694
AACGCAG7100.028.4575186
Produced by FastQC (version 0.11.2)