FastQCFastQC Report
Sun 14 Apr 2019
SRR6576345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482024
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13270.275297495560387No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7620.15808341493369624No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6960.1443911506481005No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6430.13339584750966757No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6180.12820938376512372No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5310.11016048993411118No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4840.10040993809436874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG30650.037.2608341
TACATGG30700.037.047062
ACATGGG30800.036.6215973
GTATCAA12800.034.881121
GGTACCT5250.033.1222048
GGGTACC5400.033.0724687
TGGGTAC5800.031.6019136
CATGGGT8500.031.5161174
ACGTAGG1351.3244062E-831.33181294
ATGGGTA5950.030.8052255
ATGGGAG5350.030.7461725
CATGGGG14150.030.224684
AACGCAG15950.028.5816846
CAACGCA16100.027.7315735
TCAACGC16100.027.4396614
ATCAACG16150.027.3547083
TATATAG1057.415238E-526.8558395
GTCTTAG2305.456968E-1226.5639271
TTATGCA1956.039045E-1026.5115324
GCCTTAT2008.094503E-1025.8487451
Produced by FastQC (version 0.11.2)