FastQCFastQC Report
Sun 14 Apr 2019
SRR6576345_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576345_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482024
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12750.26450965097173584No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8270.17156822066951022No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7230.14999253149220784No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6700.13899722835377493No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6560.13609280865683038No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5750.11928866612450832No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5730.11887374902494481No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4840.10040993809436874No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4830.100202479544587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.037.9225921
GTACATG28750.037.692761
GTATAAG1152.7030183E-936.8733521
TACATGG29550.036.584812
ACATGGG29600.036.3642163
TATAAGC550.002714451634.1843832
CATGGGG15500.033.65714
ATGGGTA4950.032.2819025
TTAACTC600.00415707931.3356863
CATGGGT6800.030.410894
GGGTACC5100.029.4893517
CATTACA1154.1873027E-628.6108422
AACGCAG16150.028.5193066
GGTACCT5300.028.3765478
TCAACGC16200.028.1411694
CAACGCA16450.027.9991955
TGGGTAC5550.027.9451456
ATCAACG16250.027.7682383
GTACATT856.712889E-427.7152651
GTATAGG856.712889E-427.7152651