FastQCFastQC Report
Sun 14 Apr 2019
SRR6576346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433345
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12500.2884537723984354No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7180.16568784686566132No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6620.1527651178622114No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6230.1437653601633802No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5940.13707323264373653No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5900.13615018057206152No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4800.11076624860099919No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4640.10707404031429922No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4620.10661251427846173No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4590.10592022522470548No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4480.10338183202759925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAG500.001702601737.592151
TACATGG27000.036.3780862
GTACATG27200.036.1064341
ACATGGG27300.035.45783
TAATACT550.002717929434.1746834
GTATCAA12000.031.7183781
CATGGGG13500.030.6306424
TTAGGAT1005.3235533E-528.1941154
TGGGTAC5400.027.8460396
AACGCAG13350.027.806936
GGTACCT5250.027.7465888
GGGTACC5250.027.7465887
CATGGGT7350.027.4908584
ATGGGTA5300.027.4848275
GTATATA1057.421623E-526.8515361
TCAACGC14100.026.66114
CAACGCA14200.026.4733475
TTTACAC1606.9090675E-826.4319823
ATGGGGG4650.026.2740865
TATTAGT909.513255E-426.1086752