FastQCFastQC Report
Sun 14 Apr 2019
SRR6576346_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576346_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433345
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13340.3078378659036103No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7370.17007234420611753No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6890.1589957193460176No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6560.1513805397546989No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6150.14191925602003022No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5870.13545789151830528No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5060.11676608706688667No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4960.11445845688769919No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4910.11330464179810543No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4730.10915090747556795No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4600.10615098824262423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4550.10499717315303049No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4490.103612595045518No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4440.10245877995592426No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4340.10015114977673678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA5450.038.7985235
GTACATG28900.037.9410441
GGTACCT5600.037.7854428
TACATGG29150.037.7204172
TGGGTAC5800.037.26746
GGGTACC5700.037.0968367
ACATGGG30000.036.181783
GTATCAA13150.033.6397931
GACTAAG702.1664874E-433.614231
CATGGGT7200.033.2841034
ATGGGAG5950.031.5894625
CTAACGT300.004165048731.32621852-53
CATGGGG14300.029.9022984
TATATAC1154.1958756E-628.6021983
GTCTTAG1853.2196112E-1027.9815751
GTACCTG7750.027.9097639
ACGTAGG1205.8058795E-627.432694
AACGCAG16250.027.1815196
ATGGGGG5750.026.9677875
CATATAT1057.347083E-526.8913841
Produced by FastQC (version 0.11.2)