FastQCFastQC Report
Sun 14 Apr 2019
SRR6576347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428691
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.1376282683797887No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5060.11803373525453066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10850.041.185351
GTACTAG952.0965672E-839.6109471
ATCAACG13100.033.0073473
GGTACCT2450.032.611968
CAACGCA13600.032.1394275
TCAACGC13500.032.0293544
AACGCAG13800.032.0142146
CCGAACT600.004141681431.3586671
ACGTTCC650.00615018428.92282594
TACGCTC650.00615018428.92282594
TATCAAC15400.028.6880632
GTACATG20950.027.6165811
CTAGACC1205.8323785E-627.4164283
ACATGGG20500.027.282693
ACGCAGA16350.027.0211727
TGTATCG700.00883032926.8569099
GGGCGTA350.00883516826.85690956-57
TACATGG21100.026.7296242
CGCAGAG16850.026.2193578
GGGTACC3050.026.1964937
Produced by FastQC (version 0.11.2)