FastQCFastQC Report
Sun 14 Apr 2019
SRR6576347_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576347_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428691
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.15232416822373224No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4470.10427090841655179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10500.046.1918371
CAACGCA12500.037.972285
ATCAACG12500.037.5963173
AACGCAG13100.036.591836
TCAACGC12950.036.289884
TATCAAC14200.033.769072
GTACATG21050.033.107541
TACATGG21050.032.606792
ACATGGG22200.030.2718073
ACGCAGA15950.030.053487
CGCAGAG16050.029.866238
GTATAGG1154.127238E-628.6627271
CATGGGT5150.028.288494
GTACTTG2800.026.907871
CGTTGTA350.008833989526.85764380-81
GCAGAGT18800.025.4975019
CATGGGG8550.024.7344174
CTAGTAC1351.4557772E-524.3679813
GAGTGCG1000.001757890623.4976987
GTGTAAC1050.00230768222.4232231
Produced by FastQC (version 0.11.2)