FastQCFastQC Report
Sun 14 Apr 2019
SRR6576350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213214
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3410.15993321264082097No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA2570.12053617492284746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA3900.063.8706781
TATACCG250.005230569756.3990145
CAACGCA6050.043.503375
AACGCAG6400.042.5930066
TCAACGC6200.041.6928184
ATCAACG6400.040.389923
CGCAGAG7350.036.4483458
ACGCAGA7500.036.346037
GTACAAG1055.079528E-835.80891
GTACATG10650.035.304551
TATCAAC7100.035.0838932
TACATGG10700.034.2610862
CATATAT550.002712302834.181223
ATAGTGT550.002712302834.181226
ACATGGG10900.033.6324423
CCTAAAG600.004153808631.3327882
GCAGAGT8700.031.3327879
CATGGGG7150.028.265244
GGGGATA700.00882093126.8566725
TCCGAAC350.00883065426.85667274-75
Produced by FastQC (version 0.11.2)