FastQCFastQC Report
Sun 14 Apr 2019
SRR6576351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173636
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3810.21942454329747285No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2030.11691123960469027No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2020.11633532216821396No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA1840.10596880831164045No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1770.10193738625630629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA301.4533307E-678.311491
GTAATAT301.3697878E-462.6491971
GATCTAA405.671126E-446.9868971
TATACAG608.807611E-539.1557465
AAACGAT500.001700377737.5895165
TAATGCC500.001700377737.5895164
GTATCAA3350.036.467441
TAAACGA550.002714397234.1722874
CTTTATA550.002714397234.1722871
CCTTATG702.1809692E-433.562072
ATAAACG600.004157016531.3245983
ACGCCGC753.26953E-431.3245966
ATATATA753.26953E-431.3245962
CAACGCA4050.030.1644275
TCAACGC4050.030.1644274
TATATAC650.00614835328.915013
TAACGCC650.00614835328.915014
AACGCCG650.00614835328.915015
GTACATG8800.028.8328691
ATCAACG4250.028.7449233
Produced by FastQC (version 0.11.2)