FastQCFastQC Report
Sun 14 Apr 2019
SRR6576351_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576351_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173636
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4040.23267064433642792No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2700.1554977078486028No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2550.1468589463014582No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1920.11057614780345089No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1790.10308922112925892No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA1760.10136146881982999No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAT250.005231114656.393083
GCGTACA250.005231114656.393082
CTTAGGT601.5985643E-646.994243
TAGGTAT555.2683194E-542.7220345
GTACATG8300.041.971011
TATAGTA901.2974851E-841.796739
GTATAGT901.2974851E-841.796738
TACATGG8850.039.8256262
GTATCAA3700.039.441711
ACATGGG8800.038.4498333
GGTACCT1800.036.572148
GTCTTAG801.1403628E-535.3066941
ACTAGCA550.002712318434.1776282
GGGTACC1800.033.9402857
TTAGGTA702.1789684E-433.567314
AACGCAG4400.033.1095776
GTACCTG2000.032.9149259
CAACGCA4500.032.373815
TGGGTAC1606.002665E-1132.308546
TCAACGC4550.032.018054
Produced by FastQC (version 0.11.2)