FastQCFastQC Report
Sun 14 Apr 2019
SRR6576353_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576353_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155903
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7560.48491690345920224No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3810.24438272515602652No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.19435161606896598No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2300.1475276293592811No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2210.14175480908000487No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2070.13277486642335298No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2060.13213344194787785No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1920.12315349929122596No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1690.10840073635529784No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1640.10519361397792217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTATC250.005231392656.3896067
TCTATAC250.005231392656.3896063
CTAGTAC405.666691E-446.991343
CTCGATA205.675472E-446.9913464-65
TAGTACT405.666691E-446.991344
GGTTAGC555.2478314E-542.7468228
TGGGTTA555.267752E-542.7194026
ATGGGTA1450.042.1301655
GTATCAA8600.041.1126751
CTGTGCG704.594993E-640.3041429
GTACATG12650.038.012161
GGGTACC1251.4915713E-1037.5930757
ATGGGGC2000.037.593075
GCTCGAT250.001701665337.5930764-65
TACATGG12650.037.5187762
AACGCAG9650.036.0348136
ACATGGG12800.035.9777453
TCAACGC9950.034.9483344
ATCAACG9900.034.6501773
CAACGCA9950.034.476065