FastQCFastQC Report
Sun 14 Apr 2019
SRR6576354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439658
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9600.21835153687639028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.13828930668838052No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5170.11759140058863934No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5160.11736395107105976No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5050.11486200637768447No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4750.10803852085029728No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4620.10508167712176282No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4490.10212483339322838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11000.044.449381
GTACATG30950.042.6845471
ACATGGG30350.042.4295773
TACATGG30900.042.2971732
CATGGGA15000.037.91154
CATGGGG11950.034.215924
TACCGTG550.002715836534.1801647
CATGGGT6850.033.618814
ATGGGAG5600.033.5698055
ATCAACG13950.033.353223
TCAACGC13950.033.353224
ATGGGTA4400.033.1120345
CAACGCA14350.033.0785375
AACGCAG14450.032.849626
GAGTGCG600.004159198631.3318169
GGGTACC4050.030.1713777
ATGGGAT5250.029.5414285
TTCGAAA804.777491E-429.3735799
ATGGGGG4200.029.093835
CCGAACT650.006141297528.9315471