FastQCFastQC Report
Sun 14 Apr 2019
SRR6576354_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576354_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439658
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7950.18082236647576072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.13715205910048264No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5850.13305796778405032No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5140.11690905203590063No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4710.10712872277997898No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4450.10121503532291008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12650.049.1144331
TGCGACG250.001702093337.59872422-23
TCAACGC16450.037.1542744
AACGCAG17050.036.398286
ATCAACG16850.036.2722743
GTACTAG651.4001105E-436.2061541
CAACGCA16850.035.9932565
GTACATG27700.035.8532371
ACATGGG27950.035.1557043
TACATGG28800.034.6078572
TATCAAC18900.032.337982
CATGGGT6100.031.5998764
ACGCAGA19800.030.857547
CATGGGA14800.030.1781924
CGCAGAG20250.029.7076348
TAGGTAT1354.61132E-727.8604155
CTAACTC856.797122E-427.6555583
ATGGGTA3650.027.049415
GCTTATA1057.395842E-526.8653982
GCAGAGT22850.026.7386999
Produced by FastQC (version 0.11.2)