FastQCFastQC Report
Sun 14 Apr 2019
SRR6576355_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576355_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394721
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7440.18848756463426067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.15352616151661555No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5850.148205947998713No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4940.12515168942113544No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4620.11704469739385541No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4250.10767098786231288No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3980.10083071333929536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.046.5131071
GTACATG29500.044.841661
ACATGGG30250.043.3416333
TACATGG30700.043.318612
ATGGGTA4850.039.7254265
CATGGGT8150.038.055094
CATGGGA15200.037.408324
AACGCAG15550.036.8685386
ATCAACG15800.036.285173
TCAACGC15850.036.1707084
CATGGGG10400.035.2442054
CAACGCA16300.035.172135
GGGTACC3450.034.0523727
ATGGGAT5300.033.692575
ACGCAGA17250.033.2351157
CGCAGAG18250.031.9532838
GGTACCT3700.031.7756888
ATGGGAC3700.031.7515345
ATGGGAG5650.031.6054235
TATCAAC19150.030.1830252