FastQCFastQC Report
Sun 14 Apr 2019
SRR6576358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405341
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5230.12902716478224507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.12705351790221073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGA200.00216239470.496486
TACCGTA250.00523463556.3971867
GTATCAA8050.050.830121
ATCAACG9400.043.5031973
CAACGCA9750.042.4236345
TCAACGC9750.041.9415474
AACGCAG10450.040.0267146
TATCAAC11600.035.252592
GTATAGA1405.0567905E-1033.594621
ACGCAGA12500.033.462337
CTAGTAC600.00415633831.3356343
GTACATG18300.030.3269481
CGCAGAG13650.030.2988538
CCGAACT953.7287293E-529.7047181
ACATGGG19050.029.6084753
TACATGG19400.029.0742992
GCAGAGT15050.027.4803569
CTATAAG1406.313021E-726.8756941
CATGGGT5100.025.8058154
ATTACAC1659.279938E-825.6382483
Produced by FastQC (version 0.11.2)