FastQCFastQC Report
Sun 14 Apr 2019
SRR6576359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168452
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.2303326763707169No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3600.21371073065324245No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2040.12110274737017074No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2010.11932182461472704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.11754090185928336No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1960.1163536200223209No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1800.10685536532662122No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1750.1038871607342151No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1730.10269987889725263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGA250.00522830856.3999983
GACCGTG352.9303043E-453.7142877
GTATCAA8450.043.3846171
GGACCGT450.001011490441.777786
GTACATG17300.038.849711
TACATGG17400.038.626442
CATGGGA10250.038.5170754
ACATGGG17700.037.9717523
ATGGGAG4250.037.65
TATAAAG801.1527976E-535.252
TGGGAGA2050.034.3902446
CATGGGC2500.033.844
AACGCAG10450.033.732066
TCTTAGG702.1771267E-433.571432
ATCAACG10700.032.9439243
TCAACGC10800.032.638894
CAACGCA10900.032.3394475
TCATGTA753.2637804E-431.3333322
GTATATA753.2637804E-431.3333321
TATCAAC11550.030.519482