FastQCFastQC Report
Sun 14 Apr 2019
SRR6576359_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576359_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168452
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.27248118158288415No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3440.2042124759575428No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2460.14603566594638234No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2340.1389119749246076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.1306010020658704No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1980.11754090185928336No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1810.10744900624510247No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1700.10091895614180894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACT352.9318122E-453.7086944
GTATCAA8550.042.9492151
CACTCTA1251.4915713E-1037.596089
TACATGG17100.036.276922
GTACATG17350.036.089341
AACGCAG10800.034.8111846
ACATGGG17550.034.543413
CACGTCT550.002711847234.1782574
ATCAACG10750.034.0987743
TCAACGC10750.034.0987744
CAACGCA11000.033.751035
ATGGGAT3150.032.8219765
CATGGGA12000.032.113324
ATGGGAG3150.031.3300695
TATCAAC11900.030.8035142
TTGAACA1701.200533E-1030.4085964
CGCAGAG12700.029.9732558
TAATCAT953.7361606E-529.6811185
ACGCAGA12950.029.394627
AACACTC1602.1082087E-929.371947
Produced by FastQC (version 0.11.2)