FastQCFastQC Report
Sun 14 Apr 2019
SRR6576360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158296
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.2451104260372972No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3440.217314398342346No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2470.1560367918330217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.1332945873553343No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1940.1225552130186486No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1890.11939657350785869No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1870.11813311770354272No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1740.10992065497548896No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1690.10676201546469906No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1620.10233992014959316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC451.6012167E-552.2571872
AAACGCA451.6102356E-552.2076573
GCAAACG555.241626E-542.755881
GTACATG17700.040.9200331
TACATGG18000.040.2380332
ATGGGAT2950.039.8193975
CATGGGC2150.039.337864
ACATGGG18350.038.152843
GACGGTA500.001699924437.589518
CATGGGA10700.036.0086444
TGGGATC959.402902E-734.6219186
TACGACG550.002713676534.1722835
ACGGTAT550.002713676534.1722839
TGGGATA851.7486167E-533.1672176
ATGGGAG3550.031.7657835
GTTATAT600.00413666431.3543111
ATGGGCG753.2682356E-431.324595
CGACGGT600.004155919431.324597
ACGACGG600.004155919431.324596
CATGGGT3200.030.8351464