FastQCFastQC Report
Sun 14 Apr 2019
SRR6576361_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576361_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296519
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4920.16592528640660464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.13186338818085855No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3010.10151120164306503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC200.002160592770.506583
GTATCAA7850.044.4072381
ACATGGG17850.038.4461673
GTACATG18400.038.4029921
TACATGG18450.036.9411162
ATCAACG10300.035.5955543
AACGCAG10500.034.917546
CAACGCA10550.034.7520565
TCAACGC10600.034.1446954
CATGGGT4000.034.0781824
ATGGGTA2050.032.1005555
TCGTAGA600.00415415131.3362562
TATCAAC11300.031.1976012
ACGCAGA11950.030.2873447
CGCAGAG12150.030.1705678
ATGGGGA3850.029.3014355
CATGGGA10450.029.2371794
CATGGGG7150.028.9257774
ATGGGAG4800.028.3984815
ATGGGCG856.800369E-427.649645
Produced by FastQC (version 0.11.2)