FastQCFastQC Report
Sun 14 Apr 2019
SRR6576362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272901
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5420.19860682078849107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.12935093678660026No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3140.11506004008779741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.048.1653751
AACGCAG9700.038.752616
TACATGG16700.038.2793272
ATCAACG10250.037.5900343
ACATGGG16800.037.4781573
GTACATG17250.037.331321
TCAACGC10650.036.6193964
CAACGCA10700.035.5700075
GCAAACG550.00271204634.1852841
ATGGGAG3900.033.7346425
GTATAAC702.1793562E-433.5748331
ACGCAGA11200.033.5625277
CGCAGAG11400.032.9737138
TATCAAC12000.032.1199232
CATGGGA9950.032.112094
ATGGGTA2550.031.325035
CATGGGG5600.030.2062744
CTATAGT650.006154024528.915414
GCAGAGT13550.028.0884919
TGCCCGC856.7986443E-427.6498621
Produced by FastQC (version 0.11.2)