FastQCFastQC Report
Sun 14 Apr 2019
SRR6576363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295694
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9480.3206017031120009No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5320.17991572368732542No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5170.17484291192922413No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4830.1633445386108612No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4360.1474497284354772No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3870.13087854335901303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.11532192063416911No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3300.11160185867822817No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3290.11126367122768809No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3280.110925483777148No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3190.10788179672228723No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3130.10585267201904672No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3090.10449992221688638No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3020.10213261006310578No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3010.10179442261256569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC555.252968E-542.755582
GTATCAA8050.042.065121
ACTAGAC450.001012804541.777183
TACATGG16550.041.4897042
GTACATG16850.041.309251
ACATGGG17200.039.8947753
CATGGGA5750.038.416844
ATCAACG10000.033.8395163
ATGGGAG3250.033.2610635
AACGCAG10150.032.876376
CAACGCA10450.032.3823135
TCAACGC10550.032.0753674
GGCGTAC600.00414257631.3540921
CATGGGG8350.030.9576384
CATGGGT4900.029.7342664
ACGCAGA11250.029.6617957
GCAAACG804.7539183E-429.3944631
AAACGCA804.772798E-429.3745773
GTATACA650.00612701428.942241
GGTACCT3600.028.721818
Produced by FastQC (version 0.11.2)