FastQCFastQC Report
Sun 14 Apr 2019
SRR6576363_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576363_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295694
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8390.2837392710031316No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5010.16943191272058275No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4580.1548898523473591No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4400.14880247823763756No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3850.13020216845793287No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3830.1295257935568527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.12884941865577254No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3550.1200565449417303No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3470.11735104533740963No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3360.11363098338146868No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3270.11058729632660791No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3190.10788179672228723No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2990.10111804771148553No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2980.10077986026094543No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2970.10044167281040535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7400.041.3807221
GTACATG16350.037.169641
TACATGG16550.036.349542
ACATGGG16450.036.28483
GTACTAG651.3916136E-436.2387351
ATGGGTA3300.034.1809585
GGATAGT550.002714095434.1809586
CATGGGT5700.033.8061644
CAATACT702.1814168E-433.5705834
CTCGATA300.00415987531.33254464-65
AACGCAG10150.031.023856
GGGTACC3050.030.8188957
GGTACCT2950.030.2704268
CATGGGG7600.029.6834624
CAACGCA10150.029.6347225
ATCAACG10200.029.4894543
CTAGTAC650.00614763428.9223483
TCAACGC10450.028.7839624
TGGGTAC3200.027.9055486
TATGGGT856.8050384E-427.646364
Produced by FastQC (version 0.11.2)