FastQCFastQC Report
Sun 14 Apr 2019
SRR6576364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268310
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8950.3335693787037382No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5540.20647758190153181No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4530.16883455704222727No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4480.16697104096008347No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4290.1598896798479371No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3900.14535425440721553No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3440.12820990645149266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.11702880995862995No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3130.11665610674220117No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3040.11330177779434238No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2950.10994744884648354No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2910.1084566359807685No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2870.1069658231150535No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2840.10584771346576721No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2820.10510230703290971No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2820.10510230703290971No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2750.10249338451790839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT558.769184E-751.286561
CTCGCAA405.676992E-446.98639394
GTATCAA7650.044.861771
AACGCAG9100.038.2087176
ATAACGC500.00170212237.5891153
GTACATG16550.037.2124521
ATCAACG9350.036.6845663
TACATGG16950.036.0569232
TCAACGC9550.035.9163064
ACATGGG17050.035.8254053
CAACGCA9600.035.729245
CATGGGG7750.034.5577354
CCTACTA550.00271716934.1719259
ACGCAGA10350.033.1401637
CGCAGAG10950.032.182468
AAGGCCG902.5916983E-531.3242655
ATGGGTA3400.030.402965
TGCCCGC1452.6371708E-829.1802851
TACTTAG650.00615457528.9147055
TATCAAC11900.028.8397122
Produced by FastQC (version 0.11.2)