FastQCFastQC Report
Sun 14 Apr 2019
SRR6576364_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576364_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268310
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9070.3380418173008833No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4930.1837426856993776No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4910.18299727926652007No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4520.1684618538257985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.15951697663150832No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3910.1457269576236443No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3840.143118035108643No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3670.1367820804293541No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3350.12485557750363387No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3080.11479259066005741No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3020.11255637136148484No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2960.11032015206291229No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2900.10808393276433975No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2850.10622041668219596No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2840.10584771346576721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA250.005234430556.3925067
GTATCAA9150.040.157541
GTACATG16750.034.8738981
TACATGG16800.034.4061432
TAGTATG550.002715082434.177284
ACATGGG16900.033.3683473
CATGGGG7100.032.4323124
TCAACGC11450.031.6027894
AACGCAG11250.031.3291726
ATCAACG11750.031.1958563
CAACGCA11700.030.5258585
CTTAGGG650.00614986728.9192353
ACGCAGA12600.027.9724757
TATAGAG856.8076653E-427.6433872
ATGGGTA2900.027.5480635
CGCAGAG13050.027.0230148
GTATATA1406.212049E-726.9187891
GGTACCT2800.026.8685958
GGCGTAA350.00881355226.86859568-69
TATCAAC14000.026.5179062
Produced by FastQC (version 0.11.2)