FastQCFastQC Report
Sun 14 Apr 2019
SRR6576365_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576365_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337512
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7040.20858517623077105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.12266230534025456No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3960.11732916162980872No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3690.10932944606413994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3510.10399630235369409No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3470.10281115930692836No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3440.10192230202185405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATA450.001013062541.7767222
ATACGTG450.001013062541.7767225
TACATGG19700.039.603062
GTACATG20300.038.7559171
ACATGGG20700.037.2357753
ATGGGAG4200.036.9276435
GTAGTAA550.002683225834.262171
CATGGGG7450.030.9119744
GTATCAA10300.029.7299181
CATGGGT5250.029.5421124
CCTAATA650.00607795628.9910641
CTAACCC650.006148853328.9223443
CATGGGA11000.028.6265534
GTACAAT1057.288858E-526.9202771
CCGATAC700.00882842826.85646694
ATGGGTA3050.026.196065
AACGCAG12150.025.530226
ATGGGGG3550.025.1542955
GTACTTT9750.024.6424081
ATCAACG12600.024.245423
Produced by FastQC (version 0.11.2)