FastQCFastQC Report
Sun 14 Apr 2019
SRR6576366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306931
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7560.24630943110992373No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4270.13911921571949395No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4190.13651276671303975No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4130.13455792995819907No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4000.13032245032271098No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.11142569502591787No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3420.11142569502591787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG23600.035.2626571
CATGGGT4600.034.7289544
ACATGGG23950.034.1361353
TACATGG24000.034.0872342
CCCATAT600.004148974531.3445841
ATGGGTA2650.030.142115
GTTATAG650.00613646228.9334621
CTAGACA650.006156041828.9146064
CATGGGA12000.028.191744
GTATCAA13050.028.1020371
GGGTACC2900.027.5436527
GGTACCT2750.027.3374468
ATGGGAG4000.027.0170825
AACGCAG13900.026.7044076
CATCTAG909.4818353E-426.1204851
CATGGGG8750.025.2383174
AGACCCG950.001304633724.7295975
TTAACCC950.001304633724.7295974
TGGGTAC3250.024.5774156
GTCTTAA1351.44888345E-524.379121
Produced by FastQC (version 0.11.2)