FastQCFastQC Report
Sun 14 Apr 2019
SRR6576366_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576366_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306931
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7220.23523202283249328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4380.14270308310336852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.1342321238323923No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3980.12967083807109742No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3810.12413213393238219No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3620.11794181754205342No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3200.10425796025816877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC500.001701691537.5927664
ATGGGAG3500.034.9075665
CATGGGT4350.033.4878124
TACATGG21750.033.487812
GTACATG22300.032.7205161
ACATGGG21700.032.6987763
ACGAGCG300.004163402631.32730790-91
AGGGCGA650.006153017728.9175135
ATGGGTA2700.027.8464955
GTATATG856.7412015E-427.6913641
GTATCAA12100.027.6227111
CGTGCGG350.00884116426.85197422-23
CATGGGC3700.026.6705444
GGGTACC2500.026.3149387
CATGGGG8100.025.5259514
AACGCAG13300.024.7320826
GTATTAA1151.3591386E-424.5610371
GGTACCT2501.6370905E-1124.4512318
CATGGGA11350.024.4270174
GTATAAT1751.6128251E-724.2101671
Produced by FastQC (version 0.11.2)