FastQCFastQC Report
Sun 14 Apr 2019
SRR6576369_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576369_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271731
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.2484074323503759No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2980.10966728124505486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.10561916012527095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10200.042.0833051
GTAATAC702.1815332E-433.5690653
TCAACGC12450.033.218544
CAACGCA12600.032.8230825
ATCAACG12600.032.8230823
AACGCAG13000.032.1746566
TATCAAC14150.030.2351472
TACATGG16400.029.527082
CTTAGAC1303.317855E-728.921043
GTACATG17200.028.2473281
ACATGGG17200.027.5968913
ACGCAGA14850.027.5334157
CGCAGAG15200.027.2086098
GTTCTAG909.300397E-426.2057231
CATGGGG6650.024.7350984
GCAGAGT16950.024.6767259
AGTACTT8600.023.7715812-13
AGAGTAC15650.023.27311910-11
CATGGGA10850.021.657464
AGTAAGG1100.003048334521.3699952
Produced by FastQC (version 0.11.2)