FastQCFastQC Report
Sun 14 Apr 2019
SRR6576372_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576372_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373921
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11560.30915621214106725No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7380.19736789321808615No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5920.15832221244594447No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5490.14682245715003972No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5290.14147373375659564No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4770.12756705293364107No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4470.11954396784347496No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4440.11874165933445835No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4360.11660216997708073No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4320.1155324252983919No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4270.11419524445003089No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4210.11259062743199767No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4060.1085790848869146No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4000.1069744678688814No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3950.10563728702052037No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3940.10536985085084817No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3880.10376523383281495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT5750.043.3207554
GTATCAA9300.040.0094261
ATGGGTA4900.038.3664745
GTACATG21750.038.112861
TACATGG21700.037.6857762
GGTACCT4450.036.965458
ACATGGG22150.036.707973
TGGGTAC4950.035.1305166
GGGTACC5100.033.1757137
GTATTAC753.230936E-431.3998031
TATTACA1102.9619168E-629.908412
CCTTATA953.7461643E-529.6835332
CATGGGG8600.029.5109544
TGTACTG2101.8189894E-1229.0945765
AACGCAG12600.029.0945746
GTACCTG5850.028.922429
ATCAACG12750.028.3836673
TCAACGC12850.028.1627854
CAACGCA12950.027.945315
GTGTATG2007.876224E-1025.9048391
Produced by FastQC (version 0.11.2)